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Evaluation of an enzyme-linked immunosorbent assay for direct detection of respiratory syncytial virus in nasopharyngeal aspirates.
A total of 3430 nasopharyngeal aspirates (NPAs) were tested simultaneously for the presence of respiratory syncytial virus (RSV) by direct immunofluorescence (DFA) and by an enzyme-linked immunosorbent assay (ELISA) for virus-induced major outer membrane protein, the A antigen. A total of 197 (5.6%) DFA-positive samples were detected, and further study by ELISA demonstrated that only 62 of these 197 samples were positive. The results from ELISA were positive in 51 of the NPAs that were DFA negative; 30 of these were further confirmed by plaque-reduction neutralization assay as non-virus-specific reactive antibodies. These results indicate that DFA is a sensitive test that detects both virus and antibody; however, it cannot differentiate between virus and non-virus-specific antibody. The ELISA detects virus and virus-specific antibody only; therefore, the ELISA will always detect genuine virus-specific antibody, but it is of no value for discriminating between virus and non-virus-specific reactive antibody. of the series’ best, the first nine issues of “Green Arrow” are now collected in a single volume.

John’s op-ed appeared in The Sun,

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